mRNA vaccines and therapeutics

Simplify your approach to in vitro transcription (IVT) with opDNA® templates. Featuring an open-ended 3’ end, opDNA® eliminates the need for enzymatic linearization, unlike traditional plasmid DNA or other synthetic templates. The linear open format of opDNA® and the absence of a bacterial backbone contribute to consistently
high mRNA yields.

opDNA®

opDNA strand with gradient blue and purple strands and pink end caps

opDNA® is a partially opened, linear, double stranded DNA product. Each opDNA® molecule features a hairpin on either the 3’ or 5’ end, with an opposite open end. The open end of the construct can include an overhang or blunt end.

Designed with flexibility in mind, opDNA® templates support both enzymatic and co-transcriptional capping methods. You can also encode long, continuous poly-A sequences directly into the template, removing the need for enzymatic tailing. Resulting in a well-defined poly-A tails and greater consistency in your final mRNA product. 

Benefits of using opDNA®

  • Increased mRNA yields with less DNA input compared to pDNA

  • No need for linearization, 3’ open end feeds directly into IVT processes

  • Increased PolyA stability up to ~180 length

  • Achieve high mRNA purity

  • Suitable for manufacture of long sequences +20 kb, including self-amplifying mRNAs

What we offer

  • Rapid synthesis of custom opDNA® templates including larger samRNA templates suitable for use in GMP IVT processes

  • Off-the-shelf reporter mRNA constructs for testing and validation

  • Advanced polyA analytics

  • IVT platform support to help you optimize your processes

  • LNP platform support

  • Available in RUO, HQ and GMP grade

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Technology

Find out more about our enzymatic manufacturing process and the benefits of synthetic DNA.

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Manufacturing

Discover our RUO, HQ, and GMP-grade synthetic DNA, designed for every stage, from discovery to commercial production