Quality Control

Absence of endonucleases and exonucleases:

4BB™ MagniPhi® DNA polymerase has been determined to be free of detectable endonucleases, exonucleases and nicking activity. A fluorogenic substrate designed to react with all these kinds of nucleases has been incubated in the presence of 1μg of polymerase enzyme for one hour at 37°C. No increase in fluorescence compared with the negative control was detected.

Absence of ribonucleases:

4BB™ MagniPhi® DNA polymerase has been determined to be free of detectable single-strand ribonuclease activity. A fluorogenic substrate designed to react with these kinds of nucleases has been incubated in the presence of 1μg of the enzyme for 30 min at 37°C. No increase in fluorescence compared with the negative control was detected.

Purity:

The purity of the enzyme has been determined to be higher than 95% by SDS polyacrylamide gel electrophoresis and densitometric measurements.

Functional assay

4BB™ MagniPhi® DNA polymerase has been tested for exponential amplification of 10pg and 1ng of high molecular weight human genomic DNA. In the absence of DNA input (non-template control, NTC) no background amplification was exhibited under similar conditions.

Shipping and Storage

4BB™ MagniPhi® DNA  polymerase is shipped on dry ice. On receipt, the kit should be stored immediately at -20oC in a non-frost-free (constant temperature) freezer. When stored correctly, the product can be kept for a minimum of six months after shipping without displaying any reduction in performance. For longer periods, store the kit at -80oC. Avoid repeated freeze-thaw cycles.