Box of TruePrime Apocketal Cell-Free DNA Amplification Kit with a small sample tube nearby.

4BB™ TruePrime® Apoptotic Cell-Free DNA Amplification Kit

Pack Size

5 Reactions

SKU: 340005

20 Reactions

SKU: 340020

If you want to evaluate this product, please contact us at info@4basebio.com

Product information

The 4BB™ TruePrime® Apoptotic Cell-Free DNA Amplification Kit is optimized to exponentially amplify low input cell-free DNA (160-170bp) derived from apoptotic cells in liquid biopsy samples. One of the main challenges in cell-free DNA analysis is the limited amount of DNA obtained from bodily fluids, which affects the number and type of tests that can be performed. Further challenges are posed by short DNA fragment lengths, as well as the damaged nature of apoptotically-derived DNA. See how the 4BB™ TruePrime® Apoptotic Cell-Free DNA Amplification Kit provides solutions to these challenges.

Diagram illustrating the process of cell-free DNA pretreatment, involving the removal of protruding ends, addition of 3' dA overhangs, and ligation of hairpin adaptors for some form of DNA amplification or analysis.

Using multiple displacement amplification (MDA), accurate isothermal DNA amplification takes place, using cell-free DNA obtained from plasma, serum, urine, CSF or any other bodily fluids.

Note: This kit cannot be used for the amplification of DNA from necrotic cells (≥1kb). For this we would recommend the 4BB™ TruePrime® necrotic cell-free / exosomal DNA amplification kit.

Diagram illustrating the process of DNA amplification using PCR. It shows the cycle of denaturation, primer annealing, and extension, including the addition of hairpin adaptors, primer recognition, strand displacement, and exponential amplification.

4BB™ TruePrime® Apoptotic Cell-Free DNA Amplification Kit

Product information

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