Set of laboratory test tubes with colored caps labeled for DNA testing, placed next to a box of necrotic cell-free/exosomal DNA amplification kit.

4BB™ TruePrime® Necrotic Cell-Free / Exosomal DNA Amplification Kit

The 4BB™ TruePrime® Liquid Biopsy Kit amplifies genomic cell free DNA obtained from plasma, serum, urine or CSF. It allows amplification of circulating tumour DNA to permit sequencing with a low initial input of DNA and enhances the sensitivity of cell free DNA analysis.

  • Excellent sensitivity and reproducibility

  • Does not generate primer artefacts

  • Excellent coverage.

Pack Size

25 Reactions

SKU: 330025

If you want to evaluate this product, please contact us at info@4basebio.com

Product information

The 4BB™ TruePrime® Necrotic Cell-Free / Exosomal DNA Amplification kit preferentially amplifies larger (≥1kb) DNA fragments derived through necrosis or from extracellular vesicles like exosomes. This presents a method for assessing cancer genome information regardless of tumour, with superior sensitivity (100 pg cfDNA input) and specificity.

Note: This kit cannot be used for the amplification of apoptotic cell-free DNA (160-170 bp). For this we would recommend the 4BB™ TruePrime® apoptotic cell-free DNA amplification kit.

Venn diagram comparing SNV detection in tumor biopsy samples using TruePrime®-amplified, cell-free DNA and non-amplified, cell-free DNA, showing overlaps of 488, 380, 824, 451, and 369 SNVs.

Applications:

  • Assessing cancer genome information from all samples, regardless of tumour, with superior sensitivity and specificity

  • Genetic research using large (≥1kb) circulating cell-free DNA

  • Cancer research

  • Amplification of large (≥1kb) DNA

Docuements

Protocol 4BB TruePrime Necrotic Cell-Free/Exosomal DNA Amplification Kit

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MSDS 4BB TruePrime Necrotic Cell-Free/Exosomal DNA Amplificaiton Kit

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